sox2 mouse cell signaling technologies 4900s  (Cell Signaling Technology Inc)

Journal: Journal of chemical neuroanatomy

Article Title: Neuronal expression of SOX2 is enriched in specific hypothalamic cell groups.

doi: 10.1016/j.jchemneu.2014.09.003

Figure Lengend Snippet: Fig. 2. Conservation of authenticated hypothalamic SOX2 expression in old adult rats and mice. (A) Immunoblot analysis using the rabbit anti-SOX2 antibody to demonstrate specific detection of an approximately 34 kDa protein band that is enriched in nuclear (N) compared with cytoplasmic (C) brain hippocampus protein extracts (25 mg/lane). Note that SOX2 protein in the hippocampal extracts reflects the abundant (non-neuronal) expression of SOX2 in the subgranular zone of the dentate gyrus. (B and C) Representative fluorescence microscopic images of P50 (B) and P293 (C) rat brain showing immunohistochemically detected SOX2+ cells that are abundant in the SCN. The image in (B) was obtained with the mouse anti-SOX2 antibody (L1D6A2, see the ‘Material and methods’ section). This was used to verify SOX2 localization obtained with the rabbit antibody (used for all other images). Scale bars = 50 mm. (D and E) Representative fluorescence microscopic images of P50 rat brain showing sections processed in parallel for immunohistochemical detection of Sox2 where the primary antibody was omitted in (E). Scale bars = 100 mm. (F) Representative fluorescence microscopic image of mouse brain showing a similar pattern of SOX2 expression in the mouse hypothalamus. Scale bar = 100 mm. Abbreviations as Fig. 1. CP, choroid plexus; GCL, granule cell layer; LV, lateral ventricle; SGZ, subgranular zone of the dentate gyrus; SVZ, sub-ventricular zone.

Article Snippet: The following primary antisera were used: (rabbit) antiEGR1 (1/250; 15F7, Cell Signalling Technology, Beverly, MA, USA), (rabbit) antiGFAP (1/400; G560AA, Promega, Madison, WI, USA), (mouse) anti-MeCP2 (1/200; 4B4, Active Motif, Carlsbad, CA, USA), (mouse) anti-NeuN/Fox-3 (1/100; MAB377, Millipore, Temecula, CA, USA), (rabbit) anti-SOX2 (1/1000; 39823, Active Motif), (mouse) anti-SOX2 (1/100; L1D6A2, Cell Signalling Technology).

Techniques: Expressing, Western Blot, Immunohistochemical staining